白头翁汤对内毒素诱导血管内皮细胞基因表达谱的影响

doi:10.11843/j.issn.0366-6964.2013.10.023

Abstract

Abstract:

A high-throughput and efficient Affymetrix rat genome array was used to investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatillae Decoction (PD), used for the treatment of diseases induced by lipopolysaccharide (LPS). Endothelial cell was challenged with 1 μg·mL^－1 LPS for 3 h, then treated with PD at a concentration of 1 mg·mL^-1 for 12 h. Total RNA of each treated group was extracted from cultured endothelial cell for detection by the Affymetrix Rat Genome 230 2.0 Array. The samples from LPS group and blank control group were subjected to Real-time RT-PCR, using the chimeric fluorescence method. The results showed that in LPS group, 36 genes were upregulated and 33 genes were downregulated in comparison with Blank group; In LPS-PD group, 566 genes were upregulated and 12 genes were downregulated in comparison with LPS group, and 93 genes were upregulated and 29 genes were downregulated in comparison with Blank group. The RT-PCR ratios for the target genes Il1α, Il6, Edn1, Tnfsf11 and Ptges were 9.78, 1.76, 1.43, 2.98 and 2.12 respectively, which were very similar to the ratios determined by the gene chips (10.57, 1.53, 1.59, 3.16 and 1.71, respectively). These results indicate that PD could simultaneously reduce the translated level of inflammatory factors, suppress the transcription of apoptosis genes, accelerate the protein synthesis of organism and enhance the energy metabolism of cells, thus play the united antiendotoxin action.

CLC Number:

S853.9

HU Yi-yi, MU Xiang, HU Yuan-liang. Effects of Pulsatillae Decoction on Gene Expression of LPS-induced Endothelial Cell[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1675-1684.

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Effects of Pulsatillae Decoction on Gene Expression of LPS-induced Endothelial Cell

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